In rodent experimental diabetes models changes in these parameters were reported, but no such data are available in other mammalian species including the dog. The present study was designed to analyse the effects of experimental type I diabetes on ventricular repolarization and its underlying transmembrane ionic currents and channel proteins in canine hearts.
Methods and results: Diabetes was induced by a single injection of alloxan, a subgroup of dogs received insulin substitution. After the development of diabetes 8 weeks electrophysiological studies were performed using conventional microelectrodes, whole cell voltage clamp, and ECG. Expression of ion channel proteins was evaluated by Western blotting.
Research to stop type 1 - Diabetes research - Diabetes UK
The QT c interval and the ventricular action potential duration in diabetic dogs Were moderately prolonged. Western blot analysis revealed a reduced expression of Kv4. Conclusion: It is concluded that type I diabetes mellitus, although only moderately, lengthens ventricular repolarization, attenuates the repolarization reserve by decreasing I-to and I-Ks currents, research on diabetes mellitus thereby may markedly enhance the risk of sudden cardiac death.